STL Stem Cell Wake-up Solution for Culturing Human Epidermal Stem Cells


epidermal stem cells
STL stem cell wake-up fluid


Human skin epidermal stem cells are multipotent stem cells with unlimited proliferation potential and multidirectional differentiation ability. In this study, the skin samples were separated, the epidermal sheet was digested with Dispase II protease, the cells were resuspended, and the STL stem cell wake-up fluid was added for culture. The epidermal stem cells were identified using immunocytochemistry that detected the expression of their surface markers β1 integrin and CK19. The clone formation of epidermal stem cells and keratinocytes was compared. The results showed that the cell morphology and growth were good, and the epidermal stem cells were positively stained for β1 integrin and CK19 keratin. The clone formation rate of epidermal stem cells was significantly higher than that of keratinocytes.


Rudan MV, Mishra A, Klose C, et al., 2020, Human Epidermal Stem Cell Differentiation is Modulated by Specific Lipid Subspecies. Proc Natl Acad Sci USA, 117(36): 22173-22182.

Blanpain C, Fuchs E, 2006, Epidermal Stem Cells of the Skin. Annu Rev Cell Dev Biol, 22: 339-373.

Fuchs E, 2018, Skin Stem Cells in Silence, Action, and Cancer. Stem Cell Reports, 10(5):1432-1438.

Fujiwara H, Tsutsui K, Morita R, 2018, Multi-Tasking Epidermal Stem Cells: Beyond Epidermal Maintenance. Dev Growth Differ, 60(9): 531-541.

Mokry J, Pisal R, 2020, Development and Maintenance of Epidermal Stem Cells in Skin Adnexa. Int J Mol Sci, 21(24): 9736.

Chermnykh E, Kalabusheva E, Vorotelyak E, 2018, Extracellular Matrix as a Regulator of Epidermal Stem Cell Fate. Int J Mol Sci, 19(4): 1003.

Flora P, Ezhkova E, 2020, Regulatory Mechanisms Governing Epidermal Stem Cell Function During Development and Homeostasis. Development, 147(22): dev194100.

Nanba D, Toki F, Tate S, et al., 2015, Cell Motion Predicts Human Epidermal Stemness. J Cell Biol, 209(2): 305-315.

Eckert RL, Adhikary G, Balasubramanian S, et al., 2013, Biochemistry of Epidermal Stem Cells. Biochim Biophys Acta, 1830(2): 2427-2434.

Quist SR, Eckardt M, Kriesche A, et al., 2016, Expression of Epidermal Stem Cell Markers in Skin and Adnexal Malignancies. Br J Dermatol, 175(3): 520-530.

Jackson CJ, Tønseth KA, Utheim TP, 2017, Cultured Epidermal Stem Cells in Regenerative Medicine. Stem Cell Res Ther, 8(1): 155.

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